Western Blot:Article Title: Elevated SLC40A1 impairs cardiac function and exacerbates mitochondrial dysfunction, oxidative stress, and apoptosis in ischemic myocardia
Article Snippet: .. Supplier Flag Mouse WB: 1/1000 MA1-91878 Thermo Fisher Scientific, USA IP: 1/100 Flag Rabbit WB: 1/2000 ab205606 Abcam, UK SLC40A1 Rabbit WB: 1/1000 NBP1-21502 Novus, USA SLC40A1 Mouse WB: 1/1000 ab239583 Abcam, UK IHC: 1/100 IF: 1/100 Steap4 Rabbit WB: 1/500 11944-1-AP Proteintech, China IP: 1/50 IHC: 1/50 Steap4 Goat WB: 1/500 NB100-68162 Novus, USA IF: 1/100 Fth Rabbit WB: 1/1000 A19544 ABclonal, China Hif2-α Rabbit WB: 1/1000 A7553 ABclonal, China Cox-IV Rabbit WB: 1/5000 11242-1-AP Proteintech, China Cytochrome c Rabbit WB: 1/5000 ab133504 Abcam, UK β-Actin Rabbit WB: 1/5000 81115-1-RR Proteintech, China Gpx4 Rabbit WB: 1/1000 A11243 ABclonal, China 1 Table S3 2 shRNA target sequences were as follows: 3 Target Species Target sequences Slc40a1 Mouse GTGGATCCATCCTTAGTATTT Steap4 Mouse CAGAGTCAAATGCGGAATA Slc40a1 Rat TCTTACACAAGAATGAGCTTCTGAA (1) Slc40a1 Rat TAACATCCGTGAACTTGAATGTGAA (2) Slc40a1 Rat CGAGATGGATGGGTCTCCTACTATA (3) 4 Table S4 5 Mass spectrum analysis of Flag (SLC40A1)-interacting proteins 6 Protein name Unique peptides Molecular mass (kDa) Abundance MS score SLC40A1 10 62.7 2.3 × 108 41 Steap4 5 53 3.7 × 106 9 Tfrc 5 85.7 1.5 × 106 4 Ndufs4 2 19.8 8.5 × 105 6 Abcb7 2 82.5 2.4 × 105 0 Following Co-IP assays of TG mouse heart tissue and electrophoresis, the whole band (Flag-interacting 7 proteins) assessed by LC-MS/MS. ..
Immunohistochemistry:Article Title: Elevated SLC40A1 impairs cardiac function and exacerbates mitochondrial dysfunction, oxidative stress, and apoptosis in ischemic myocardia
Article Snippet: .. Supplier Flag Mouse WB: 1/1000 MA1-91878 Thermo Fisher Scientific, USA IP: 1/100 Flag Rabbit WB: 1/2000 ab205606 Abcam, UK SLC40A1 Rabbit WB: 1/1000 NBP1-21502 Novus, USA SLC40A1 Mouse WB: 1/1000 ab239583 Abcam, UK IHC: 1/100 IF: 1/100 Steap4 Rabbit WB: 1/500 11944-1-AP Proteintech, China IP: 1/50 IHC: 1/50 Steap4 Goat WB: 1/500 NB100-68162 Novus, USA IF: 1/100 Fth Rabbit WB: 1/1000 A19544 ABclonal, China Hif2-α Rabbit WB: 1/1000 A7553 ABclonal, China Cox-IV Rabbit WB: 1/5000 11242-1-AP Proteintech, China Cytochrome c Rabbit WB: 1/5000 ab133504 Abcam, UK β-Actin Rabbit WB: 1/5000 81115-1-RR Proteintech, China Gpx4 Rabbit WB: 1/1000 A11243 ABclonal, China 1 Table S3 2 shRNA target sequences were as follows: 3 Target Species Target sequences Slc40a1 Mouse GTGGATCCATCCTTAGTATTT Steap4 Mouse CAGAGTCAAATGCGGAATA Slc40a1 Rat TCTTACACAAGAATGAGCTTCTGAA (1) Slc40a1 Rat TAACATCCGTGAACTTGAATGTGAA (2) Slc40a1 Rat CGAGATGGATGGGTCTCCTACTATA (3) 4 Table S4 5 Mass spectrum analysis of Flag (SLC40A1)-interacting proteins 6 Protein name Unique peptides Molecular mass (kDa) Abundance MS score SLC40A1 10 62.7 2.3 × 108 41 Steap4 5 53 3.7 × 106 9 Tfrc 5 85.7 1.5 × 106 4 Ndufs4 2 19.8 8.5 × 105 6 Abcb7 2 82.5 2.4 × 105 0 Following Co-IP assays of TG mouse heart tissue and electrophoresis, the whole band (Flag-interacting 7 proteins) assessed by LC-MS/MS. ..
shRNA:Article Title: Elevated SLC40A1 impairs cardiac function and exacerbates mitochondrial dysfunction, oxidative stress, and apoptosis in ischemic myocardia
Article Snippet: .. Supplier Flag Mouse WB: 1/1000 MA1-91878 Thermo Fisher Scientific, USA IP: 1/100 Flag Rabbit WB: 1/2000 ab205606 Abcam, UK SLC40A1 Rabbit WB: 1/1000 NBP1-21502 Novus, USA SLC40A1 Mouse WB: 1/1000 ab239583 Abcam, UK IHC: 1/100 IF: 1/100 Steap4 Rabbit WB: 1/500 11944-1-AP Proteintech, China IP: 1/50 IHC: 1/50 Steap4 Goat WB: 1/500 NB100-68162 Novus, USA IF: 1/100 Fth Rabbit WB: 1/1000 A19544 ABclonal, China Hif2-α Rabbit WB: 1/1000 A7553 ABclonal, China Cox-IV Rabbit WB: 1/5000 11242-1-AP Proteintech, China Cytochrome c Rabbit WB: 1/5000 ab133504 Abcam, UK β-Actin Rabbit WB: 1/5000 81115-1-RR Proteintech, China Gpx4 Rabbit WB: 1/1000 A11243 ABclonal, China 1 Table S3 2 shRNA target sequences were as follows: 3 Target Species Target sequences Slc40a1 Mouse GTGGATCCATCCTTAGTATTT Steap4 Mouse CAGAGTCAAATGCGGAATA Slc40a1 Rat TCTTACACAAGAATGAGCTTCTGAA (1) Slc40a1 Rat TAACATCCGTGAACTTGAATGTGAA (2) Slc40a1 Rat CGAGATGGATGGGTCTCCTACTATA (3) 4 Table S4 5 Mass spectrum analysis of Flag (SLC40A1)-interacting proteins 6 Protein name Unique peptides Molecular mass (kDa) Abundance MS score SLC40A1 10 62.7 2.3 × 108 41 Steap4 5 53 3.7 × 106 9 Tfrc 5 85.7 1.5 × 106 4 Ndufs4 2 19.8 8.5 × 105 6 Abcb7 2 82.5 2.4 × 105 0 Following Co-IP assays of TG mouse heart tissue and electrophoresis, the whole band (Flag-interacting 7 proteins) assessed by LC-MS/MS. ..
Co-Immunoprecipitation Assay:Article Title: Elevated SLC40A1 impairs cardiac function and exacerbates mitochondrial dysfunction, oxidative stress, and apoptosis in ischemic myocardia
Article Snippet: .. Supplier Flag Mouse WB: 1/1000 MA1-91878 Thermo Fisher Scientific, USA IP: 1/100 Flag Rabbit WB: 1/2000 ab205606 Abcam, UK SLC40A1 Rabbit WB: 1/1000 NBP1-21502 Novus, USA SLC40A1 Mouse WB: 1/1000 ab239583 Abcam, UK IHC: 1/100 IF: 1/100 Steap4 Rabbit WB: 1/500 11944-1-AP Proteintech, China IP: 1/50 IHC: 1/50 Steap4 Goat WB: 1/500 NB100-68162 Novus, USA IF: 1/100 Fth Rabbit WB: 1/1000 A19544 ABclonal, China Hif2-α Rabbit WB: 1/1000 A7553 ABclonal, China Cox-IV Rabbit WB: 1/5000 11242-1-AP Proteintech, China Cytochrome c Rabbit WB: 1/5000 ab133504 Abcam, UK β-Actin Rabbit WB: 1/5000 81115-1-RR Proteintech, China Gpx4 Rabbit WB: 1/1000 A11243 ABclonal, China 1 Table S3 2 shRNA target sequences were as follows: 3 Target Species Target sequences Slc40a1 Mouse GTGGATCCATCCTTAGTATTT Steap4 Mouse CAGAGTCAAATGCGGAATA Slc40a1 Rat TCTTACACAAGAATGAGCTTCTGAA (1) Slc40a1 Rat TAACATCCGTGAACTTGAATGTGAA (2) Slc40a1 Rat CGAGATGGATGGGTCTCCTACTATA (3) 4 Table S4 5 Mass spectrum analysis of Flag (SLC40A1)-interacting proteins 6 Protein name Unique peptides Molecular mass (kDa) Abundance MS score SLC40A1 10 62.7 2.3 × 108 41 Steap4 5 53 3.7 × 106 9 Tfrc 5 85.7 1.5 × 106 4 Ndufs4 2 19.8 8.5 × 105 6 Abcb7 2 82.5 2.4 × 105 0 Following Co-IP assays of TG mouse heart tissue and electrophoresis, the whole band (Flag-interacting 7 proteins) assessed by LC-MS/MS. ..
Electrophoresis:Article Title: Elevated SLC40A1 impairs cardiac function and exacerbates mitochondrial dysfunction, oxidative stress, and apoptosis in ischemic myocardia
Article Snippet: .. Supplier Flag Mouse WB: 1/1000 MA1-91878 Thermo Fisher Scientific, USA IP: 1/100 Flag Rabbit WB: 1/2000 ab205606 Abcam, UK SLC40A1 Rabbit WB: 1/1000 NBP1-21502 Novus, USA SLC40A1 Mouse WB: 1/1000 ab239583 Abcam, UK IHC: 1/100 IF: 1/100 Steap4 Rabbit WB: 1/500 11944-1-AP Proteintech, China IP: 1/50 IHC: 1/50 Steap4 Goat WB: 1/500 NB100-68162 Novus, USA IF: 1/100 Fth Rabbit WB: 1/1000 A19544 ABclonal, China Hif2-α Rabbit WB: 1/1000 A7553 ABclonal, China Cox-IV Rabbit WB: 1/5000 11242-1-AP Proteintech, China Cytochrome c Rabbit WB: 1/5000 ab133504 Abcam, UK β-Actin Rabbit WB: 1/5000 81115-1-RR Proteintech, China Gpx4 Rabbit WB: 1/1000 A11243 ABclonal, China 1 Table S3 2 shRNA target sequences were as follows: 3 Target Species Target sequences Slc40a1 Mouse GTGGATCCATCCTTAGTATTT Steap4 Mouse CAGAGTCAAATGCGGAATA Slc40a1 Rat TCTTACACAAGAATGAGCTTCTGAA (1) Slc40a1 Rat TAACATCCGTGAACTTGAATGTGAA (2) Slc40a1 Rat CGAGATGGATGGGTCTCCTACTATA (3) 4 Table S4 5 Mass spectrum analysis of Flag (SLC40A1)-interacting proteins 6 Protein name Unique peptides Molecular mass (kDa) Abundance MS score SLC40A1 10 62.7 2.3 × 108 41 Steap4 5 53 3.7 × 106 9 Tfrc 5 85.7 1.5 × 106 4 Ndufs4 2 19.8 8.5 × 105 6 Abcb7 2 82.5 2.4 × 105 0 Following Co-IP assays of TG mouse heart tissue and electrophoresis, the whole band (Flag-interacting 7 proteins) assessed by LC-MS/MS. ..
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